In this experiment u r testing three variables (pH, temp and substrate concentration). You cant test three independent variables with two experiments. You need to do three experiments to test all those variables. e.g. for the pH experiment. Set up 8 test tubes. Add an equal amount of substrate and enzyme to every tube. Then add an equal amount of an acidic or basic solution of different pH's to every tube, so that one tube has a pH of 2, one with 3, one with 4....up till 9 or whatever. Keep the tubes at the same temp for the same time period. At the end, note the level of enzymatic action on the substrate, and then make conclusions based on what was the optimum pH to catalase that substrate. You would then do two more; one with a varying temperature (everything else is kept equal) and one with a varying substrate level.Hi there, ive been given an assessment task to create two experiments from an experiment that i did earlier during the week.the experiment was the effect of substrate concentration on the action of an enzyme. using the experiment as a model, i have to write the aim and method for two experiments to test the effect of:increased temperature and change in pHon the enzyme peroxidase and the substrate hydrogen peroxide.can anyone help me with this? as i am not quite sure how to do it.
ummm. Yeah, i suppose.Could the pH solution be HCL?