There are multiple interpretations for the last stanza:
*It could be that he took the unconventional path.
*On the other hand, the poem was written as a gentle satire on a friend of Frost's who spent all his time regretting the direction he took at crossroads, so it could be poking fun at him for placing too much importance on such a trivial thing rather than enjoying the moment. This is likely because he says at about 3 other points in the poem that both roads are the same ("equally lay", "really about the same" and another one I don't remember)
*Or, he could be thinking about the choice he made, and explaining that his choice had either a) bad long-term effects or b) good long term effects (both can be sort of the same as the first interpretation, i.e. unconventional path was good/bad for him.. but it could be just a metaphor for choice generally rather than common or uncommonly taken paths)
The title could be significant as meaning
a) The road I didn't take (regretful tone, thinking about what he's missed - again, satire of his friend)
b) The road I didn't take (just plain regret, or alternatively, *not* having made a significant mistake)
c) The road less people took (I took the less conventional, normally not-taken road)
Hope that helps everyone. There are points for and against all those views, it just depends on how *you* see it really. As long as you can use language in the poem to back up your opinion about it, you're good.