ummm..
just one point of clarification.. according to the syllabus, its the relationship between temperature and enzyme activity and the relationship between substrate concentration and enzyme activity.. not reaction rate as u say.. these are different terms.
substrate concentration:
catalase is an enzyme that catalyses the reaction H2O2 --> H2 + O2
thus enzyme activity can be compared (which is in essence, 'investigating') by measuring relative amounts of Oxygen bubbles produced.. generally the more substrate present (potato), the greater the enzyme activity (
upto a certain point, called saturation point beyond which it does not increase further because all active sites of enzymes are in use).. note the term is not 'used up' but 'in use' as enzymes are 're-usable'
temperature:
as in all investigations, given that other conditions are 'controlled', enzyme activity increases with increasing temperature. the greater the temperature of the medium, the more of the 'effect' or 'reaction' can be observed..again, upto a certain point, beyond which, activity plummets dramatically due to the denaturation of the enzyme (structure of enzyme breaks down).
i suggest that u have a look through the theory of a practical investigation, and identify why different steps are done, and then do it. good luck
